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Thermostabilization of BSA in TMAO Water Mixtures by Infrared Spectroscopy

2019

Background:Trimethylamine-N-Oxide (TMAO) is a small organic molecule derived from the metabolism of L-carnitine and choline after ingestion of animal food. TMAO has many functions such as electron acceptor, an osmolyte, stabilizer of macromolecules folding. It seems that TMAO plays an important role in nature but, in humans, it is a remnant of the evolution of the osmolyte system.Objective:The present paper is addressed on the study of thermal stability of hydrated Bovine Serum Albumins (BSA) in the presence of water and TMAO water solution by means of InfraRed spectroscopy. In particular, this work has investigated the protein amide I spectral regions, which is sensitive to protein seconda…

0303 health sciencesBSAChemistryTMAO BSA infrared spectroscopy spectral shift spectral distance wavelet cross correlation.Analytical chemistryInfrared spectroscopyTMAOspectral distancespectral shift03 medical and health sciences0302 clinical medicinewavelet cross correlation030220 oncology & carcinogenesisinfrared spectroscopy030304 developmental biologyCurrent Chemical Biology

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